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GPCA Funded Grant - Cancer


 

 

Announcement

Two New GPCA Funded Grants

Completed Grant -  The GPCA Funded Osteosarcoma Grant


Completed Grant

GPCA Funded Grant

Broad Institute (Non-profit Organization)
Active Grant No: 373A:
Mapping Genes Associated with Osteosarcoma in Large Dog Breeds
Disease(s):

Cancer

Sponsor(s):

Borzoi Club of America, Forsyth Kennel Club, Golden Retriever Foundation, Great Pyrenees Club of America, Great Pyrenees Club of Puget Sound, Greyhound Club of America, Irish Setter Club of America Foundation, Irish Wolfhound Club of America, Inc., Newfoundland Club of America Charitable Trust, Rhodesian Ridgeback Club of the United States, Rottweiler Health Foundation, St. Bernard Club of America, Starlight Fund

Researcher(s):

Kerstin Lindblad-Toh, PhD

Breed(s):

Borzoi, Golden Retriever, Great Pyrenees, Greyhound, Irish Setter, Irish Wolfhound, Newfoundland, Rottweiler, Saint Bernard

Abstract:

Eight thousand to 10,000 cases of a malignant bone tumor called osteosarcoma are reported in dogs in the United States annually, representing a significant health concern. In the majority of cases, spread of the tumor through the body and death follows within a few years. Osteosarcomas affect all dogs, but the disease frequency is considerably higher in large and giant breeds, including the long-limbed hounds (Irish Wolfhound, Great Dane, Greyhound, Scottish Deerhound, Rhodesian Ridgeback, Great Pyrenees and Borzoi) and mastiff-type breeds (Rottweiler, Labrador Retriever, Flat-Coated Retriever, Golden Retriever, Mastiff, Bullmastiff, Saint Bernard, Irish Setter, and Newfoundland). It is clear the genetics plays an important role. We propose to identify the genetic risk factors for osteosarcoma in two breeds: Greyhound and Rottweiler. While certain characteristics of these two breeds make them ideal to study, we expect that the genes identified in these breeds may also be associated with osteosarcoma in related breeds. This study should lead to the development of genetic tests for osteosarcoma that could be used to eliminate carriers from breeding populations, eventually reducing the frequency of this devastating cancer. Ultimately, it could also lead to improvements in treatment of osteosarcoma.

 

For more information in participating - contact Flo Laicher @ pyrshire@comcast.net


 

Cancer(s) Affecting The Great Pyrenees

 

Type of Cancer(s) affecting the Great Pyrenees vary, the most common however, is Osteosarcoma, with Hemangiosarcoma rising behind it.
 

The GPCA currently co-funds the Grant below with Broad Institute for Osteosarcoma.   This is a very important study and the Researchers are seeking samples of both "affected" and "non-affected" Great Pyrenees.  

The Broad Institute is also conducting a Study on various types of Cancer - The Dog Genome Project.     They are also seeking samples of both "affected" and "non-affected" Great Pyrenees for the following Cancers.

Osteosarcoma   Hemangiosarcoma
     
Melanoma   Lymphoma
     
Mammary Cancer   Mast Cell

Information from the Broad Institute

Broad Institute - Contact / Instructions for Submissions for both The Dog Genome Project and Grant 373A - Osteosarcoma are below:

Please send blood samples to our new address.

  1. Collecting Samples
  2. Shipping Samples
  3. More Information

Information for veterinarians

We are unable to reimburse for the cost of shipping or veterinary expenses, and we greatly appreciate your assistance with our research.

Please see our flyer for more details on our research. If you have questions please contact us at dog-info@broad.mit.edu

Collecting Samples

We prefer blood samples because they give us more DNA and much higher quality DNA.

Unfortunately, we can not provide blood collection kits. To send a blood sample, you will need:

  1. 5mL EDTA tube
  2. the consent form signed by the owner
  3. shipping materials

Please have your vet collect 5 mL of blood in the EDTA tube. Your vet should be able to provide the EDTA tube. Click here for more information for veterinarians.

Please label the tube with the following information:

  • Dog's name
  • Breed
  • AKC number
  • Sex

If your dog has been sick, even if they are now healthy, please include copies of any diagnostic reports from your vet, including:

  • histopathology reports
  • blood tests
  • radiograph and ultrasound analysis

Shipping Samples

Ship the blood and the signed consent form to:

Dog Genome Project
Broad Institute
7 Cambridge Center
Cambridge, MA 02142

If possible, let us know to expect your sample:

E-mail: dog-info@broad.mit.edu
Fax: (617) 324-4987

When shipping blood samples, please be very careful to package it to prevent breakage and leaking. Tubes should be wrapped in soft material to pad the glass tubes, and then sealed in a ziploc bag (which will prevent any leaking if a sample tube is broken). Samples from multiple dogs may be shipped together but please ensure that the tubes are labeled clearly so that there will not be any confusion as to which dog the blood belongs to. Please use a box for the shipping that will not be crushed through normal handling by the post office (or any other carrier). Extra packing (i.e. some crumpled newspaper) should be added to the box to minimize movement of the tubes within the shipping box.

Please note that we must have a signed consent form for every dog participating in our research. We will also need the AKC number or pedigree information.

Additional Information

Many veterinarians are willing to collect additional blood for our research as part of routine bloodwork.

Even if less than 5 mL of blood is collected, please send the sample. Blood is very rich in DNA and as little as 2mL can be sufficient.

If sending blood for multiple dogs with the same owner, the owner information can be filled out on just one consent form. It is critical, though, that for each dog we have a consent form with the dog's information and the owner/agent signature.

If you have questions please contact Broad  at:
E-mail: dog-info@broad.mit.edu

or Contact Flo Laicher @ pyrshire@comcast.net

 

Update(s)

 

Update – 12/06 - submitted by Dianne Migas

Currently there are a total of 33 Samples that have been received by the Broad Institute for Great Pyrenees.    Of these 33 samples, 7 were affected with Osteosarcoma and 26 were Healthy.   The sexes for these submissions are 10 Females, 21 Males and 2 unknown.     Four Males (three neutered) were affected with Osteosarcoma,  One female was affected and Two, sexes unknown were affected.   In addition the information received from the Broad Institute (see Table), Color of all the affected are “White”. 

There was no data received as to when the dog’s where first diagnosed (date samples received) or the “age” of onset, protocol of treatment or death. 

The table below is exactly what the GPCA Health Committee received from the Researcher.

This information is also being incorporated into The Dog Genome Project.

 

 

Color / Markings

Sex

DOB

Result

White

M (N)

0

Osteosarcoma

White

M (N)

0

Osteosarcoma

White

F

2004

 

White / Blaireau

F

2003

 

White / Tan Markings

F

2003

 

White / Badger

F

2003

 

White

F

2003

 

White / Badger

M

2002

 

White / Tan Markings

F

2002

 

White / Badger

F

2002

 

White / Blaireau

F

2002

 

White / Blaireau

M

2002

 

White / Badger

F

2001

 

White

M

1998

 

White / Badger

F

1996

 

White / Blaireau

M

2003

 

White

F

1997

Osteosarcoma

White

M (N)

1998

Osteosarcoma

White / Badger

F

2005

 

White / Badger

F

2004

 

White / Tan Markings

F

2004

 

White / Tan Markings

F

2003

 

White / Tan Markings

F

2002

 

White

F

2002

 

White

F

2002

 

White / Tan Markings

M

2000

 

White

M

2004

 

White

F

2000

 

White

F

1999

 

White

F

1996

 

White

 

1996

Osteosarcoma

White

 

1996

Osteosarcoma

White

M

2000

Osteosarcoma

 

 


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